Gukurikirana isesengura ryamazi afite ibintu byinshi byifashishwa mubumenyi bwubuzima no gukurikirana ibidukikije

Gukurikirana isesengura ryamazi afite ibintu byinshi byifashishwa mubumenyi bwubuzima no gukurikirana ibidukikije.Muri iki gikorwa, twateje imbere fotometeri yoroheje kandi ihendutse ishingiye ku cyuma cyitwa waveguide capillaries (MCCs) kugirango tumenye ultrasensitive yo kwinjirira.Inzira ya optique irashobora kwiyongera cyane, kandi ndende cyane kurenza uburebure bwumubiri bwa MWC, kubera ko urumuri rwatatanye kuruhande rwicyuma cyoroshye rushobora kuba ruri muri capillary utitaye kumpande zibyabaye.Kwishyira hamwe nkibice 5.12 nM birashobora kugerwaho ukoresheje reagent zisanzwe za chromogeneque bitewe nuburyo bushya butari umurongo wa optique amplification hamwe no guhinduranya byihuse hamwe na glucose.

Photometrie ikoreshwa cyane mugusesengura ibyitegererezo byamazi bitewe nubwinshi bwibintu bya chromogenic biboneka hamwe na semiconductor optoelectronic ibikoresho1,2,3,4,5.Ugereranije na cuvette gakondo ishingiye ku kwinjirira, capillaries yamazi (LWC) yerekana (TIR) ​​mugukomeza urumuri rwimbere muri capillary1,2,3,4,5.Nyamara, nta yandi mananiza, inzira ya optique yegereye gusa uburebure bwumubiri bwa LWC3.6, kandi kongera uburebure bwa LWC burenga 1.0 m bizagira ikibazo cyumucyo mwinshi hamwe ningaruka nyinshi ziterwa n’ibibyimba, nibindi.3, 7. Kubireba kumurongo uteganijwe kugaragazwa na selile yo guhitamo inzira nziza, imipaka yo gutahura itezwa imbere gusa na 2.5-8.9.

Kuri ubu hari ubwoko bubiri bwingenzi bwa LWC, aribwo capillaries ya Teflon AF (ifite indangagaciro yo kugabanuka ya ~ 1.3 gusa, iri munsi y’amazi) hamwe na silika capillaries yashizwemo na Teflon AF cyangwa firime yicyuma1,3,4.Kugirango ugere kuri TIR kuri interineti hagati yibikoresho bya dielectric, ibikoresho bifite indangantege nkeya kandi bingana n’umucyo mwinshi birasabwa3,6,10.Kubijyanye na capillaries ya Teflon AF, Teflon AF irahumeka kubera imiterere yayo3,11 kandi irashobora gukuramo ibintu bike mubitegererezo by'amazi.Kuri capillaries ya quartz yometse hanze hamwe na Teflon AF cyangwa ibyuma, indangagaciro yo kwangirika ya quartz (1.45) irarenze ibyitegererezo byinshi byamazi (urugero 1.33 kumazi) 3,6,12,13.Kuri capillaries zometseho firime yicyuma imbere, imitungo yo gutwara abantu yarigishijwe 14,15,16,17,18, ariko inzira yo gutwikira iragoye, hejuru ya firime yicyuma ifite imiterere itoroshye kandi yuzuye4,19.

Byongeye kandi, ubucuruzi bwa LWCs (AF Teflon Coated Capillaries na AF Teflon Coated Silica Capillaries, World Precision Instruments, Inc.) zifite izindi ngaruka mbi, nka: kubera amakosa..Umubare munini wapfuye wa TIR3,10, (2) T-umuhuza (guhuza capillaries, fibre, na inlet / outlet tubes) irashobora gutega imyuka myinshi10.

Muri icyo gihe, kumenya urugero rwa glucose ni ingenzi cyane mu gusuzuma diyabete, cirrhose y'umwijima n'indwara zo mu mutwe20.nuburyo bwinshi bwo gutahura nka Photometrie (harimo na spekitifotometrie 21, 22, 23, 24, 25 na colimetry kumpapuro 26, 27, 28), galvanometrie 29, 30, 31, fluorometrie 32, 33, 34, 35, optique polarimetry 36, hejuru ya plasmon resonance.37, Fabry-Perot cavity 38, amashanyarazi 39 na capillary electrophoresis 40,41 nibindi.Nyamara, bumwe murubwo buryo busaba ibikoresho bihenze, kandi kumenya glucose yibitekerezo bya nanomolar bikomeje kuba ingorabahizi (urugero, kubipimo bifotora21, 22, 23, 24, 25, 26, 27, 28, glucose yibanze cyane).imbogamizi yari 30 nM gusa mugihe Prussian yubururu nanoparticles yakoreshejwe nka mimics ya peroxidase).Isesengura rya Nanomolar glucose risabwa kenshi kubushakashatsi bwa selile yo mu rwego rwa selile nko kubuza gukura kwa kanseri ya prostate y'abantu42 hamwe n'imyitwarire ya CO2 yo gutunganya Prochlorococcus mu nyanja.